The microspectrophotometer is sometimes known as a microscope spectrophotometer as it combines an optical microscope and an extremely sensitive spectrophotometer.
Therefore, add components have specific performance limitations due to the microscope itself. An integrated instrument avoids those limitations as it is designed especially for microspectroscopy.
In a microspectrophotometer, like those made by CRAIC Technologies, the spectrophotometer is incorporated with a specially designed microscope. The microscope optics and light sources have the finest quality and able to operate in the ultraviolet, visible and near infrared regions (normal microscopes only function in the visible range).
The microscope optics and light sources have the finest quality and able to operate in the ultraviolet, visible and near infrared regions (normal microscopes only function in the visible range). Click here for info about microspectroscopy.
The spectrophotometer is built into the microscope, together with a digital imaging system, so that the maximum amount of light can be collected from the smallest samples.
As such, microspectrophotometer are very flexible tools able to measure absorbance, transmittance, reflectance, and emission (for example, fluorescence) spectra of even sub-micron sized samples. Apart from this , Go Here for more info about Leica microscope.
How Microspectrophotometer Works
- The microscope spectrometer and microspectrometer work like this: a lamp on the microscope emits white light which is focused onto the sample. The sample absorbs some wavelengths of light better than others…it all depends upon the sample chromophore’s chemical structure and surroundings.
- Since the aperture is mirrored, the majority of light is reflected to a digital imaging system. This allows you to observe the spectrophotometer aperture overlaid on the sample and makes it very easy to align the system and take spectra. Figure 1 shows the entry aperture as a black square. Simply move the point so that the square is over the sample to make measurements.
The light that is not reflected into the electronic imaging system will pass through the aperture into the spectrophotometer. The light is split into component wavelengths by an optical grating and each component’s intensity is measured by a pixel on a Charge Coupled Device or CCD sensor.